REV1 介导的跨损伤合成
中文名称
通路描述
REV1 (hREV1) 编码一种模板依赖性 dCMP 转移酶，可将 C 残基插入无碱基位点 (Lin et al. 1999, Gibbs et al. 2000)。与 DNA 损伤位点的单泛素化 PCNA 相互作用可增强 REV1 介导的跨损伤合成 (TLS) (Garg and Burgers 2005, Wood et al. 2007)。在 REV1 将 dCMP 插入到 AP 模板位点之后，TLS 由 DNA 聚合酶 zeta 复合物 (POLZ) 继续 (Hara et al. 2010, Kikuchi et al. 2010, Xie et al. 1012)。POLZ 由催化亚基 REV3L 和辅助亚基 MAD2L2 (REV7) 组成。MAD2L2 结合 REV1，从而将 POLZ 招募到 DNA 损伤位点 (Hara et al. 2010, Kikuchi et al. 2010, Xie et al. 1012)。POLZ 具有错误倾向性，并有助于与 TLS 相关的诱变 (Shachar et al. 2009, Lee et al. 2014)。POLZ 的进程性低，在掺入少于 30 个核苷酸后从 DNA 模板解离 (Nelson et al. 1996, Lee et al. 2014)。
英文描述
Translesion synthesis by REV1 REV1 (hREV1) encodes a template-dependent dCMP transferase that can insert a C residue opposite an abasic site (Lin et al. 1999, Gibbs et al. 2000). Interaction with monoubiquitinated PCNA at a DNA damage site enhances REV1-mediated translesion synthesis (TLS) (Garg and Burgers 2005, Wood et al. 2007). After REV1 incorporates dCMP opposite to the apurinic/apyrimidinic (AP) template site, TLS is continued by the DNA polymerase zeta complex (POLZ). POLZ consists of the catalytic subunit REV3L and the accessory subunit MAD2L2 (REV7). MAD2L2 binds REV1, thus recruiting POLZ to DNA damage site (Hara et al. 2010, Kikuchi et al. 2010, Xie et al. 1012). POLZ is error-prone and contributes to TLS-related mutagenesis (Shachar et al. 2009, Lee et al. 2014). POLZ has a low processivity and dissociates from the DNA template after incorporating less than 30 nucleotides (Nelson et al. 1996, Lee et al. 2014).
所含基因
16 个基因