ALKBH3介导的烷基化损伤逆转
中文名称
通路描述
ALKBH3与ALKBH2同源，是一种类似大肠杆菌α-酮戊二酸和Fe(II)依赖的AlkB二氧合酶，可氧化烷基化DNA碱基上的甲基并释放其为甲醛。与ALKBH2类似，ALKBH3在α-酮戊二酸依赖的反应中从甲基化多核苷酸中移除1-甲基腺嘌呤(1-meA)和3-甲基胞嘧啶(3-meC)，并恢复未取代的碱基。与ALKBH2类似，ALKBH3还能在DNA中修复1-乙基腺嘌呤(1-etA)残基，同时释放乙醛。虽然ALKBH2对双链DNA(dssDNA)有偏好，但ALKBH3对单链DNA(ssDNA)有偏好。在ASCSC3 DNA解旋酶存在下，ALKBH3高效修复双链DNA，该解旋酶解开双链DNA，从而为ALKBH3提供单链底物(Dango et al. 2011)。
英文描述
ALKBH3 mediated reversal of alkylation damage ALKBH3, like ALKBH2, is a homolog of E.coli alpha-ketoglutarate- and Fe(II)-dependent dioxygenase AlkB that oxidizes methyl groups on alkylated DNA bases and releases them as formaldehyde. Like ALKBH2, ALKBH3 removes 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) from methylated polynucleotides in an alpha-ketoglutarate-dependent reaction and regenerates unsubstituted bases. Like ALKBH2, ALKBH3 can also repair 1-ethyladenine (1-etA) residues in DNA with the release of acetaldehyde (Duncan et al., 2002, Lee et al. 2005). While ALKBH2 has a preference for double strand DNA (dsDNA), ALKBH3 has a preference for single strand DNA (ssDNA). ALKBH3 efficiently repairs dsDNA in the presence of ASCC3 DNA helicase, which unwinds dsDNA, thus providing the single strand substrate for ALKBH3 (Dango et al. 2011).
所含基因
4 个基因