Rev 介导的 HIV RNA 核输出
中文名称
通路描述
HIV-1 基因组包含由单个转录本编码的 9 个基因。为了病毒复制，未剪接、单剪接和完全剪接的病毒 mRNA 必须从细胞核中导出。HIV-1 的 mRNA 剪接位点效率低下，导致不完全剪接 RNA 的积累（Staffa 和 Cochrane, 1994）。在病毒生命周期的早期或在缺乏病毒 Rev 蛋白的情况下，完全剪接的病毒 mRNA（编码调节蛋白 Tat、Nef 和 Rev）从细胞核中导出，而不完全剪接的结构蛋白编码转录本则被细胞蛋白保留在细胞核内，这些细胞蛋白通常用于防止细胞前 mRNA 的核输出。未剪接和部分剪接 mRNA 的导出由病毒蛋白 Rev 介导，该蛋白与细胞共因子一起招募到 HIV-1 mRNA 序列中的 Rev 响应元件（RRE）内（Malim 等人，1990；Fischer 等人，1994）。细胞 hRIP 蛋白对于正确介导病毒 RNA 从细胞核到细胞质的 Rev 输出至关重要（Sanchez-Velar 等人，2004；Yu 等人，2005）。
英文描述
Rev-mediated nuclear export of HIV RNA The HIV-1 genome contains 9 genes encoded by a single transcript. In order for the virus to replicate, unspliced, singly-spliced and fully spliced viral mRNA must be exported from the nucleus. The HIV-1 mRNA splice sites are inefficient resulting it the accumulation of a pool of incompletely spliced RNAs (Staffa and Cochrane, 1994). In the early stages of the viral life cycle, or in the absence of the viral Rev protein, completely spliced viral mRNA which encode the regulatory proteins Tat, Nef and Rev are exported from the nucleus while the incompletely spliced structural protein encoding transcripts are held within the nucleus by cellular proteins that normally function in preventing the nuclear export of cellular pre-mRNA. Export of both unspliced and partially spliced mRNA is mediated by the viral protein Rev which is recruited, along with cellular cofactors, to the Rev Response Element (RRE) within the HIV-1 mRNA sequence (Malim et al., 1990; Fischer et al., 1994). The cellular hRIP protein is essential for correct Rev-mediated export of viral RNAs to the cytoplasm (Sanchez-Velar et al., 2004; Yu et al., 2005).
所含基因
32 个基因