RNA Pol II CTD 磷酸化与 HIV 感染期间的 CE 相互作用
中文名称
通路描述
为了促进共转录加帽，并从而限制由 RNA 聚合酶 II 产生的 RNA 的帽结构，加帽酶直接结合到 RNA 聚合酶 II 上。最大 Pol II 亚基的 C 端结构域包含其七肽重复序列上的几个磷酸化位点。加帽酶鸟苷转移酶和甲基转移酶特异性地结合在 CTD 的 Serine 5 位点磷酸化上。TFIIH 激酶亚基 Cdk7 催化该磷酸化事件，该事件发生在启动子附近。此外，已证明加帽酶与 Serine-5 磷酸化的 CTD 结合可刺激体外鸟苷转移酶活性。
英文描述
RNA Pol II CTD phosphorylation and interaction with CE during HIV infection To facilitate co-transcriptional capping, and thereby restrict the cap structure to RNAs made by RNA polymerase II, the capping enzymes bind directly to the RNA polymerase II. The C-terminal domain of the largest Pol II subunit contains several phosphorylation sites on its heptapeptide repeats. The capping enzyme guanylyltransferase and the methyltransferase bind specifically to CTD phosphorylated at Serine 5 within the CTD. Kinase subunit of TFIIH, Cdk7, catalyzes this phosphorylation event that occurs near the promoter. In addition, it has been shown that binding of capping enzyme to the Serine-5 phosphorylated CTD stimulates guanylyltransferase activity in vitro.
所含基因
27 个基因