HIV 转录起始
中文名称
通路描述
开放复合物的形成使模板链暴露给 RNA 聚合酶 II 酶的催化中心。这促进了第一个磷酸二酯键的形成，标志着转录起始。因此，TFIIB 基础转录因子从起始复合物中解离。开放转录起始复合物是不稳定的，可以变回封闭状态。在此阶段，需要 TFIIH 持续（d）ATP 水解。二核苷酸转录本与转录复合物稳定结合。解离后形成终止产物。转录复合物也对小寡核苷酸抑制敏感。与模板在位置 -1 和 +1 互补的二核苷酸也可以指导第一个磷酸二酯键的形成。该反应与基础转录因子 TFIIE 和 TFIIH 无关，且不涉及开放复合物的形成。该反应对单链寡核苷酸抑制敏感。
英文描述
HIV Transcription Initiation Formation of the open complex exposes the template strand to the catalytic center of the RNA polymerase II enzyme. This facilitates formation of the first phosphodiester bond, which marks transcription initiation. As a result of this, the TFIIB basal transcription factor dissociates from the initiation complex.The open transcription initiation complex is unstable and can revert to the closed state. Initiation at this stage requires continued (d)ATP-hydrolysis by TFIIH. Dinucleotide transcripts are not stably associated with the transcription complex. Upon dissociation they form abortive products. The transcription complex is also sensitive to inhibition by small oligo-nucleotides. Dinucleotides complementary to position -1 and +1 in the template can also direct first phosphodiester bond formation. This reaction is independent on the basal transcription factors TFIIE and TFIIH and does not involve open complex formation. This reaction is sensitive to inhibition by single-stranded oligonucleotides.
所含基因
48 个基因