NS1 介导的宿主通路效应
中文名称
通路描述
病毒 NS1 蛋白是一种核内二聚蛋白，在感染细胞中高表达并具有 dsRNA 结合活性。RNA 结合域位于蛋白的 N 端部分。NS1 RNA 结合域形成一个对称的同源二聚体，具有六螺旋折叠。突变分析表明，二聚化对于 RNA 结合至关重要。基本残基被认为与 RNA 的磷酸骨架接触，这与观察到的缺乏序列特异性一致。NS1 及其结合的 RNA 在结合时没有发生任何显著的结构变化。NS1 二聚体跨越经典 A 型 dsRNA 的次要沟槽。NS1 的非 RNA 结合部分被称为效应器结构域，包括宿主细胞 poly (A)-结合蛋白 II（PABII）和 30kDa 亚基的切割和多聚腺苷酸化特异性因子（CPSF）的结合位点。
英文描述
NS1 Mediated Effects on Host Pathways Viral NS1 protein is a nuclear, dimeric protein that is highly expressed in infected cells and has dsRNA-binding activity. The RNA-binding domain lies within the N-terminal portion of the protein. The NS1 RNA-binding domain forms a symmetric homodimer with a six-helical fold. Mutational analysis has demonstrated that dimer formation is crucial for RNA-binding. The basic residues are believed to make contact with the phosphate backbone of the RNA which is consistent with an observed lack of sequence specificity. Neither NS1 nor its bound RNA undergo any significant structural changes upon binding. The NS1 dimer spans the minor groove of canonical A-form dsRNA. The non-RNA binding portion of NS1 has been termed the effector domain and includes binding sites for host cell poly (A)-binding protein II (PABII) and the 30kDa subunit of cleavage and polyadenylation specificity factor (CPSF).
所含基因
38 个基因