（16-20）-羟基二十碳四烯酸（HETE）的合成
中文名称
通路描述
与脂氧合酶类似，细胞色素 P450（CYP）酶催化花生四烯酸的羟基化和环氧化。然而，而脂氧合酶使用活性非血红素铁直接从花生四烯酸中抽象氢原子，CYP 则含有血红素 - 铁活性位点，通过不同的机制氧化其底物。它们将花生四烯酸在 C-5 和 C-15 之间羟基化，产生脂氧合酶类羟基二十碳四烯酸（HETEs），并在 sp3 杂化的末端碳原子上添加羟基，形成一类独特的 HETEs。氧原子转移到不稳定的花生四烯酸中间体上终止反应，分别形成 HETE 或环氧二十碳三烯酸（EETs）（Capdevila 等 2000，Buczynski 等 2009，Vance 等 2008）。
英文描述
Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE) Similar to the lipoxygenases, cytochrome P450 (CYP) enzymes catalyse the hydroxylation and epoxygenation of arachidonate. However, whereas lipoxygenases use an active non-heme iron to abstract hydrogen directly from arachidonate, CYPs contain a heme-iron active site that oxidizes its substrate by a different mechanism. They hydroxylate arachidonate between C-5 and C-15 to produce lipoxygenase-like hydroxyeicosatetraenoates (HETEs) and add a hydroxyl moiety to the sp3-hybridized omega-carbons to form a unique class of HETEs. The transfer of oxygen to the unstable arachidonate intermediate terminates the reaction by forming HETE or epoxy-eicosatrienoate (EETs), respectively (Capdevila et al. 2000, Buczynski et al. 2009, Vance & Vance 2008).
所含基因
9 个基因