小干扰 RNA（siRNA）生物发生
中文名称
通路描述
小干扰 RNA（siRNA）是 21-25 个核苷酸的单链 RNA，由 DICER1 酶在含有 DICER1、一种 Argonaute 蛋白和 TARBP2 或 PRKRA（PACT）的 RISC 装载复合物中切割较长的双链 RNA 产生。通常，长双链底物来源于病毒或基因组中的重复序列，且两条链完全互补。
经 DICER1 切割后产生的 21-25 个核苷酸的双链产物被装载到一种 Argonaute 蛋白中（人类含有 4 种 Argonaute），并通过一种机制将其变为单链，该机制尚不清楚。
siRNA 装载的 AGO2 主要位于粗面内质网的胞质面，并在细胞核中也被观察到。
经 DICER1 切割后产生的 21-25 个核苷酸的双链产物被装载到一种 Argonaute 蛋白中（人类含有 4 种 Argonaute），并通过一种机制将其变为单链，该机制尚不清楚。
siRNA 装载的 AGO2 主要位于粗面内质网的胞质面，并在细胞核中也被观察到。
英文描述
Small interfering RNA (siRNA) biogenesis Small interfering RNAs (siRNAs) are 21-25 nucleotide single-stranded RNAs produced by cleavage of longer double-stranded RNAs by the enzyme DICER1 within the RISC loading complex containing DICER1, an Argonaute protein, and either TARBP2 or PRKRA (PACT). Typically the long double-stranded substrates originate from viruses or repetitive elements in the genome and the two strands of the substrate are exactly complementary.
After cleavage by DICER1 the 21-25 nucleotide double-stranded product is loaded into an Argonuate protein (humans contain 4 Argonautes) and rendered single-stranded by a mechanism that is not well characterized.
siRNA-loaded AGO2 is predominantly located at the cytosolic face of the rough endoplasmic reticulum and has also been observed in the nucleus.
After cleavage by DICER1 the 21-25 nucleotide double-stranded product is loaded into an Argonuate protein (humans contain 4 Argonautes) and rendered single-stranded by a mechanism that is not well characterized.
siRNA-loaded AGO2 is predominantly located at the cytosolic face of the rough endoplasmic reticulum and has also been observed in the nucleus.
所含基因
9 个基因