逆行转运于反式高尔基网络
中文名称
通路描述
反式高尔基网络是反式囊泡从内溶酶系统和质膜来源的停靠位点。典型囊泡包括回收驻留的反式高尔基网络蛋白如 TGOLN2（也称为 TGN46）、受体如甘露糖 -6-磷酸受体以及毒素如沙门氏菌肠毒素、霍乱毒素和蓖麻毒素，它们利用反式运输机器“搭便车”通过分泌系统返回细胞质以释放（综述于 Johannes and Popoff, 2008; Pfeffer, 2011; Sandvig et al, 2013）。这些囊泡通过夹蛋白 -AP1 依赖性方式从内吞系统转运，详细情况见“反式高尔基网络 budding 途径”（目前尚未详述）。一般来说，在锚定和融合于 TGN 之前，囊泡是无包被的。在 TGN 处，至少存在两种不同的锚定途径。一种 RAB6 依赖性途径有助于来自早期内吞途径的囊泡的融合和锚定。这些囊泡通过与其定位在 TGN 的 Golgin 锚定蛋白相互作用以及 COG 和 GARP 多亚基锚定复合物相互作用，锚定在 TGN 上（综述于 Bonafacino and Rojas, 2006; Bonafacino and Hierro, 2011; Pfeffer, 2011）。相比之下，甘露糖 -6-磷酸受体似乎通过 RAB9 和 PLIN3 依赖性途径从晚期内体转运至 TGN。囊泡通过与 RAB9 与 RHOBTB3 非典型 RHO GTP 蛋白的相互作用招募至 TGN，并通过与 TGN 定位的 Golgin 和 GARP 复合物的相互作用锚定（Perez-Victoria et al, 2008; Perez-Victoria et al, 2009; Diaz et al, 1999; 综述于 Pfeffer, 2011; Chia and Gleeson, 2014）
英文描述
Retrograde transport at the Trans-Golgi-Network The trans-Golgi network is the docking site for retrograde cargo from the endolysosomal system and the plasma membrane. Typical cargo includes recycling resident TGN proteins such as TGOLN2 (also known as TGN46), receptors such as the mannose-6-phosphate receptors and toxins like Shiga, cholera and ricin which use the retrograde trafficking machinery to 'hitchhike' back through the secretory system for release into the cytoplasm (reviewed in Johannes and Popoff, 2008; Pfeffer, 2011; Sandvig et al, 2013). These cargo are trafficked from the endocytic system in a clathrin- and AP1-dependent manner that is described in more detail in the "Trans-Golgi network budding pathway" (just not yet). In general, it appears that vesicles are uncoated prior to their tethering and fusion at the TGN. At the TGN, at least 2 distinct tethering pathways exist. A RAB6-dependent pathway contributes to the fusion and docking of vesicles from the early endocytic pathway. These vesicles, which carry cargo such as TGOLN2 and toxins, dock at the TGN through interactions with TGN-localized Golgin tethers and with the multisubunit tethering complexes COG and GARP (reviewed in Bonafacino and Rojas, 2006; Bonafacino and Hierro, 2011; Pfeffer, 2011). In contrast, mannose-6-phosphate receptors appear to traffic from late endosomes to the TGN through a RAB9- and PLIN3-dependent pathway. Vesicles are recruited to the TGN through interaction of RAB9 with the atypical RHO GTPase RHOBTB3, and tethered by virtue of interaction with TGN-localized Golgins and the GARP complex (Perez-Victoria et al, 2008; Perez-Victoria et al, 2009; Diaz et al, 1999; reviewed in Pfeffer, 2011; Chia and Gleeson, 2014)
所含基因
48 个基因