聚合酶转换
中文名称
通路描述
在引物合成后，复制因子 C 结合到起始 DNA 的 3'端以触发聚合酶转换。Pol alpha 催化活性非过程性以及复制因子 C 与引物 - 模板连接处的紧密结合，可能引起 pol alpha:引物酶复合物的周转。当 Pol alpha-引物酶引物酶复合体被从引物上置换后，增殖细胞核抗原（PCNA）结合形成“滑动夹”结构。随后，复制因子 C 解离，DNA 聚合酶 delta 结合并催化 DNA 的连续合成。
英文描述
Polymerase switching After the primers are synthesized, Replication Factor C binds to the 3'-end of the initiator DNA to trigger polymerase switching. The non-processive nature of pol alpha catalytic activity and the tight binding of Replication Factor C to the primer-template junction presumably lead to the turnover of the pol alpha:primase complex. After the Pol alpha-primase primase complex is displaced from the primer, the proliferating cell nuclear antigen (PCNA) binds to form a "sliding clamp" structure. Replication Factor C then dissociates, and DNA polymerase delta binds and catalyzes the processive synthesis of DNA.
所含基因
14 个基因