RNA Pol II CTD磷酸化及其与CE的结合
中文名称
通路描述
为了促进共转录加帽，并限制由RNA聚合酶II合成的RNA的帽结构，加帽酶直接结合到RNA聚合酶II上。RNA Pol II最大亚基的C末端结构域含有其七肽重复序列上的几个磷酸化位点。加帽酶鸟苷转移酶和甲基转移酶特异性结合于Serine 5磷酸化的CTD。TFIIH激酶亚基Cdk7催化该磷酸化事件，该事件发生在启动子附近。此外，加帽酶与Serine 5磷酸化的CTD结合可刺激体外鸟苷转移酶活性。
英文描述
RNA Pol II CTD phosphorylation and interaction with CE To facilitate co-transcriptional capping, and thereby restrict the cap structure to RNAs made by RNA polymerase II, the capping enzymes bind directly to the RNA polymerase II. The C-terminal domain of the largest Pol II subunit contains several phosphorylation sites on its heptapeptide repeats. The capping enzyme guanylyltransferase and the methyltransferase bind specifically to CTD phosphorylated at Serine 5 within the CTD. Kinase subunit of TFIIH, Cdk7, catalyzes this phosphorylation event that occurs near the promoter. In addition, it has been shown that binding of capping enzyme to the Serine-5 phosphorylated CTD stimulates guanylyltransferase activity in vitro.
所含基因
27 个基因