I 型过氧化物酶体膜蛋白导入
中文名称
通路描述
大多数过氧化物酶体膜蛋白（PMPs）通过受体 - 伴侣蛋白 PEX19 和 docking receptor PEX3 插入到过氧化物酶体膜中（Soukupova et al. 1999, Muntau et al. 2003, Fang et al. 2004, Fujiki et al. 2006, Matsuzono and Fujiki 2006, Matsuzono et al. 2006, Pinto et al. 2006, Sato et al. 2008, Sato et al. 2010, Schmidt et al. 2010, Hattula et al. 2014，综述于 Fujiki et al. 2014, Mayerhofer 2016）。PEX19 在细胞质中翻译时与 PMP 结合。PEX 19 识别 PMP 似乎依赖于 PMP 跨膜域中带正电荷的残基（Costello et al. 2017）。PEX19:PMP 复合物随后与位于过氧化物酶体膜中的 PEX3 相互作用。通过尚未明确机制，PMP 插入到过氧化物酶体膜中，PEX19 从 PEX3 解离。当前模型涉及 PMP 从 PEX19 转移到 PEX3 的疏水区域，随后 PMP 插入膜中（Chen et al. 2014，综述于 Giannopoulou et al. 2016）。该过程似乎不需要水解 ATP 或 GTP（Pinto et al. 2006）。与 PMP 不同，PEX3 是通过结合 PEX19 然后 docking 与 PEX16 插入到过氧化物酶体膜中的（Matsuzaki and Fujiki 2008）。PEX3 和 PEX16 也可以共翻译性插入到内质网膜中（Kim et al. 2006, Yonekawa et al. 2011, Aranovich et al. 2014, Hua et al. 2015, Mayerhofer et al. 2016）。该 ER 膜区域随后形成芽状结构以贡献新过氧化物酶体。PEX3 也被观察到插入到线粒体外膜中（Sugiura et al. 2017）。ER 膜和线粒体外膜的区域随后释放以形成前过氧化物酶体囊泡，这些囊泡融合形成新过氧化物酶体（Sugiura et al. 2017）。因此，过氧化物酶体似乎源于现有过氧化物酶体的分裂以及从来自线粒体和 ER 的前体产生的新过氧化物酶体（Sugiura et al. 2017，综述于 Fujiki et al. 2014, Hua and Kim 2016）。
英文描述
Class I peroxisomal membrane protein import Most peroxisomal membrane proteins (PMPs) are inserted into the peroxisomal membrane by the receptor-chaperone PEX19 and the docking receptor PEX3 (Soukupova et al. 1999, Muntau et al. 2003, Fang et al. 2004, Fujiki et al. 2006, Matsuzono and Fujiki 2006, Matsuzono et al. 2006, Pinto et al. 2006, Sato et al. 2008, Sato et al. 2010, Schmidt et al. 2010, Hattula et al. 2014, reviewed in Fujiki et al. 2014, Mayerhofer 2016). PEX19 binds the PMP as it is translated in the cytosol. Recognition of the PMP by PEX 19 appears to depend on positively charged residues in the transmembrane domain of the PMP (Costello et al. 2017). The PEX19:PMP complex then interacts with PEX3 located in the peroxisomal membrane. Through a mechanism that is not yet clear, the PMP is inserted into the peroxisomal membrane and PEX19 dissociates from PEX3. A current model involves transfer of the PMP from PEX19 to a hydrophobic region of PEX3 followed by insertion of the PMP into the membrane (Chen et al. 2014, reviewed by Giannopoulou et al. 2016). The process does not appear to require hydrolysis of ATP or GTP (Pinto et al. 2006).
Unlike other PMPs, PEX3 is inserted into the peroxisomal membrane by binding PEX19 and then docking with PEX16 (Matsuzaki and Fujiki 2008). Both PEX3 and PEX16 can also be co-translationally inserted into the endoplasmic reticulum membrane (Kim et al. 2006, Yonekawa et al. 2011, Aranovich et al. 2014, Hua et al. 2015, Mayerhofer et al. 2016). This region of the ER membrane then buds to contribute to new peroxisomes. PEX3 is also observed to insert into the mitochondrial outer membrane (Sugiura et al. 2017). Regions of the ER membrane and mitochondrial outer membrane are then released to form pre-peroxisomal vesicles which fuse to form new peroxisomes (Sugiura et al. 2017). Peroxisomes therefore appear to arise from fission of existing peroxisomes and production of new peroxisomes from precursors derived from mitochondria and the ER (Sugiura et al. 2017, reviewed in Fujiki et al. 2014, Hua and Kim 2016).
Unlike other PMPs, PEX3 is inserted into the peroxisomal membrane by binding PEX19 and then docking with PEX16 (Matsuzaki and Fujiki 2008). Both PEX3 and PEX16 can also be co-translationally inserted into the endoplasmic reticulum membrane (Kim et al. 2006, Yonekawa et al. 2011, Aranovich et al. 2014, Hua et al. 2015, Mayerhofer et al. 2016). This region of the ER membrane then buds to contribute to new peroxisomes. PEX3 is also observed to insert into the mitochondrial outer membrane (Sugiura et al. 2017). Regions of the ER membrane and mitochondrial outer membrane are then released to form pre-peroxisomal vesicles which fuse to form new peroxisomes (Sugiura et al. 2017). Peroxisomes therefore appear to arise from fission of existing peroxisomes and production of new peroxisomes from precursors derived from mitochondria and the ER (Sugiura et al. 2017, reviewed in Fujiki et al. 2014, Hua and Kim 2016).
所含基因
19 个基因