Sealing of the nuclear envelope (NE) by ESCRT-III

Sealing of the nuclear envelope (NE) by ESCRT-III During telophase, the double membrane of the reforming NE, which is derived from fenestrated sheets and tubules of the mitotic ER, is sealed to reestablish the nucleocytoplasmic permeability barrier (reviewed by Otsuka and Ellenberg 2018). Some of the holes in the reforming nuclear envelope close around forming nuclear pore complexes (NPCs) (reviewed by Otsuka and Ellenberg 2018). Other fenestrations are sealed by the formation of filamentous ESCRT-III assemblies and their disassembly by the AAA+ ATPase VPS4 (VPS4A/VPS4B) (reviewed by Schoneberg et al. 2017). The ESCRT-III/VPS4 machinery has a general role in “reverse topology” membrane scission (i.e., involving the fusion of cytoplasmic membrane surfaces (reviewed by Schoneberg et al. 2017). In concert with these events, microtubules connected to the kinetochore and to other chromosomal regions are severed (reviewed by Schoneberg et al. 2017).