通过同源重组（HRR）
中文名称
通路描述
同源定向修复（HDR）通过同源重组（HRR）是同源重组修复（HRR）的别称。HRR 发生在 DNA 双链断裂（DSB）末端广泛切除之后，形成长的 3'-ssDNA 突出端。RAD51 在 BRCA2 的调控下覆盖 3'-ssDNA 突出端，形成侵入性的 RAD51 核丝。RAD51 核丝侵入姐妹染色质 DNA 双链，导致 D-环形成。在 D-环被 DNA 修复合成延伸后，形成的延伸 D-环或双 Holliday 结重组中间体可通过产生交叉互换或产生非交叉互换的过程进行解决（综述 Ciccia 和 Elledge 2010）。
英文描述
Formation of intermediate mesoderm Intermediate mesoderm gives rise to the urogenital system including the gonads and all components of the kidney, with the anterior intermediate mesoderm giving rise to the ureteric epithelium and the posterior intermediate epithelium giving rise to the metanephric mesenchyme. Intermediate mesoderm, is induced by a graded level of BMP signaling between the lateral plate mesoderm (high BMP activity) and the paraxial mesoderm (low BMP activity) (inferred from mouse embryos: James and Schultheiss 2005, reviewed by Davidson et al. 2019). Specification of the intermediate mesoderm in the anterior-posterior dimension is influenced by the caudal-rostral gradient Wnt and FGF signaling and rostral-caudal gradient retinoic acid signaling (inferred from mouse embryos: Cartry et al. 2006, reviewed by Davidson et al. 2019).
In mouse, the first observed markers of intermediate mesoderm are Osr1 and Lhx1, which are also expressed in the lateral plate mesoderm. Later, Pax2 and Pax8 are expressed specifically in the intermediate mesoderm. In mouse embryos, knockout experiments indicate that Osr1 activates Lhx1 and Pax2 (Wang et al. 2005), Lhx1 activates Pax2 (Tsang et al. 2000), and Pax2 activates Lhx1 and Osr1 (Boualia et al. 2013, Ranghini and Dressler 2015, reviewed in Marcotte et al. 2013). Foxc1 and Foxc2 produced in the paraxial mesoderm repress Lhx1 and Osr1 and thereby restrict the expansion of intermediate mesoderm (Wilm et al. 2004).
In vitro, human pluripotent stem cells can be induced to form intermediate mesoderm by treatment with a Wnt agonist (CHIR99021) followed by treatment with FGF2 and retinoic acid (Lam et al. 2014).
In mouse, the first observed markers of intermediate mesoderm are Osr1 and Lhx1, which are also expressed in the lateral plate mesoderm. Later, Pax2 and Pax8 are expressed specifically in the intermediate mesoderm. In mouse embryos, knockout experiments indicate that Osr1 activates Lhx1 and Pax2 (Wang et al. 2005), Lhx1 activates Pax2 (Tsang et al. 2000), and Pax2 activates Lhx1 and Osr1 (Boualia et al. 2013, Ranghini and Dressler 2015, reviewed in Marcotte et al. 2013). Foxc1 and Foxc2 produced in the paraxial mesoderm repress Lhx1 and Osr1 and thereby restrict the expansion of intermediate mesoderm (Wilm et al. 2004).
In vitro, human pluripotent stem cells can be induced to form intermediate mesoderm by treatment with a Wnt agonist (CHIR99021) followed by treatment with FGF2 and retinoic acid (Lam et al. 2014).
所含基因
8 个基因