Maturation of DENV proteins

Maturation of DENV proteins Dengue virus (DENV) uses the host to fold and cleave its polyprotein, and mature the resulting fragments. The individual steps are highly conserved in all flaviviruses. The proteins prM, E, and NS1 are glycosylated with complex glycans or high-mannose sugars (reviewed in Yap et al., 2017; Feng et al., 2022). Dengue proteins can undergo further modifications like phosphorylation, sumoylation, ubiquitination, myristoylation or hydroxyprolylation (Aviner et al., 2021; reviewed in Kumar et al., 2020; Boytz & Rolle, 2024). C and NS5 also localize to the nucleus, and NS1 is secreted to extracellular space, where they interact with host factors. NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5 form the replication complex at foci near the ER membrane which later supports budding of virions into the ER lumen (reviewed in Cleaves, 1985; Roby et al., 2015).