BMAL1（ARNTL）和 CLOCK 的磷酸化和核转位
中文名称
通路描述
昼夜节律蛋白的翻译后修饰提供额外的时相控制和代谢控制水平，以及终止转录激活和激活蛋白水解的机制（参见 Lee 等人 2001, Hirano 等人 2016, Alessandro 等人 2019）。BMAL1（ARNTL）和 CLOCK 在细胞质中合成后，CDK5 磷酸化 CLOCK（基于小鼠同源物 Kwak 等人 2013 推断），CK2α（CSNK2A1）磷酸化 BMAL1（基于小鼠同源物 Tamaru 等人 2009, Tamaru 等人 2015 推断）。磷酸化的 BMAL1 和 CLOCK 蛋白异二聚化（基于小鼠同源物 Kondratov 等人 2003, Tamaru 等人 2015 推断），并转位至细胞核（基于小鼠同源物 Tamaru 等人 2003, Kondratov 等人 2006, Kwon 等人 2006）。NPAS2 也可与 BMAL1 异二聚化（Reick 等人 2001），并假定经历类似的磷酸化，但尚未直接证实。
英文描述
Phosphorylation and nuclear translocation of BMAL1 (ARNTL) and CLOCK Post-translational modifications of circadian clock proteins provide additional levels of tempo control and metabolic control, as well as mechanisms for terminating transcriptional activation and activating proteolysis (reviewed in Lee et al. 2001, Hirano et al 2016, Alessandro et al. 2019).
After BMAL1 (ARNTL) and CLOCK are synthesized in the cytosol, CDK5 phosphorylates CLOCK (inferred from mouse homologs in Kwak et al. 2013) and CSNK2A1 (CK2alpha) phosphorylates BMAL1 (inferred from mouse homologs in Tamaru et al. 2009, Tamaru et al. 2015). The phosphorylated BMAL1 and CLOCK proteins heterodimerize (inferred from mouse homologs in Kondratov et al. 2003, Tamaru et al 2015) and are translocated to the nucleoplasm (inferred from mouse homologs in Tamaru et al. 2003, Kondratov et al. 2006, Kwon et al. 2006). NPAS2 can also heterodimerize with BMAL1 (Reick et al. 2001) and is presumed to undergo similar phosphorylation, however, this has not been directly demonstrated.
After BMAL1 (ARNTL) and CLOCK are synthesized in the cytosol, CDK5 phosphorylates CLOCK (inferred from mouse homologs in Kwak et al. 2013) and CSNK2A1 (CK2alpha) phosphorylates BMAL1 (inferred from mouse homologs in Tamaru et al. 2009, Tamaru et al. 2015). The phosphorylated BMAL1 and CLOCK proteins heterodimerize (inferred from mouse homologs in Kondratov et al. 2003, Tamaru et al 2015) and are translocated to the nucleoplasm (inferred from mouse homologs in Tamaru et al. 2003, Kondratov et al. 2006, Kwon et al. 2006). NPAS2 can also heterodimerize with BMAL1 (Reick et al. 2001) and is presumed to undergo similar phosphorylation, however, this has not been directly demonstrated.
所含基因
6 个基因