非同源末端连接
中文名称
通路描述
非同源末端连接（NHEJ）通过直接连接消除 DNA 双链断裂（DSBs）。NHEJ 涉及 KU 异二聚体与双链 DNA 末端的结合，招募 DNA-PKcs（酵母中的 MRX 复合物），末端加工，以及招募 DNA 连接酶 IV（LIG4）-XRCC4 复合物，从而实现连接。最近的研究表明，细菌仅使用两种蛋白质（Ku 和 DNA 连接酶）即可完成 NHEJ，而真核生物则需要多种因子。NHEJ 在所有细胞周期阶段修复 DSBs，无需序列同源性即可连接两个 DNA DSBs，因此是易出错的。
英文描述
Nonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) by direct ligation. NHEJ involves binding of the KU heterodimer to double-stranded DNA ends, recruitment of DNA-PKcs (MRX complex in yeast), processing of ends, and recruitment of the DNA ligase IV (LIG4)-XRCC4 complex, which brings about ligation. A recent study shows that bacteria accomplish NHEJ using just two proteins (Ku and DNA ligase), whereas eukaryotes require many factors. NHEJ repairs DSBs at all stages of the cell cycle, bringing about the ligation of two DNA DSBs without the need for sequence homology, and so is error-prone.
所含基因
13 个基因